| IL-35

Interleukin 35 (IL-35) is a recently discovered anti-inflammatory cytokine from the IL-12 family. Member of IL-12 family - IL-35 is produced by wide range of regulatory lymphocytes and plays a role in immune suppression.IL-35 can block the development of Th1 and Th17 cells by limiting early T cell proliferation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	500	250	125	62.5	31.25	15.62	7.81	0
OD	2.39	1.73	1.2	0.78	0.49	0.35	0.23	0.08
Corrected OD	2.31	1.65	1.12	0.7	0.41	0.27	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse IL-35 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	82-102	97
Plasma(n=8)	90-106	101
Cell Culture Supernates(n=8)	96-109	106

 

| Linearity

Samples were spiked with high concentrations of Mouse IL-35 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-96	87-95	90-110
	Average (%)	91	94	96
1 : 4	Range (%)	89-103	88-108	105-115
	Average (%)	94	98	108